WET LABORATORY

We operate a fully equipped laboratory to providing a consistent and high quality operation from dissection to digital reporting. Our laboratory is currently achieved by the following:

Specimen Dissection

Specimen dissection is carried out by a trained Biomedical Scientist with support from a clinical team as required.

Processing

We have two Thermo Fisher processors with programmes for;

  • Overnight Processing
  • Urgent Biopsies
  • Fatty and large Blocks
  • Weekend Program

 

Embedding

Embedding is prepared on a standard Leica embedding centre with heated forceps.

Section Cutting

All section cutting is undertaken on a Thermo Scientific Finesse Microtome for both small and large blocks.

H&E Staining

All automated staining is on our DAKO Coverstainer.

Dako CoverStainer automates every step of the H&E process from baking, dewaxing and staining through to the dehydrated, coverslipped and dried slide that is ready for examination by the pathologist.

Special Stains

We currently have offer a full range of special stain:

 

 

 

Alcian Blue

Alcian Blue PAS

Alcian Blue DPAS

Congo Red

EVG

Gram

Grocott

Hexamine

HVG

Masson Trichrome

Masson Fontana

MSB

Oil Red O

Orcein

PAS

PAS D

Perls

Retic

Von Kossa

ZN

 

Immnohistochemistry

Our IHC is carried out on  a Roche Ventana BenchMark XT

The BenchMark XT system delivers consistent, high-quality results while at the same time improving workflow, flexibility and increasing test volume. it offer a single drawer of 30 slide positions and 35 reagent and can process up to 60 slides in 8 hours, or 90 slides with an overnight run. It can simultaneous IHC, ISH, SISH, Dual Stain, and FITC slide processing

 

Beta Catenin

BerEP4

Calretinin

CD3

CD8

CD10

CD20

CD30

CD31

CD34

CD45

CD56

CD68

CDX2

Chromogranin A

CK5/6

CK7

CK20

D2-40

Desmin

EMA

ER

HMB45

H-Pylori

Melan A

MLH1

MOC-31

HSA

MSH2

P16

P53

P63

Pan Keratin

PSA

PSAP

S100

SMA

SOX10

Synaptophysin

TFF3

TTF1

Vimentin

WTI

 

Note: Antibodies in bold are those on  our UKAS ISO15189 schedule, we are working on getting all antibodies added.

We are happy to expand our repertoire on request.

Slide Scanning

We have a IV-CE accredited Hamamatsu slide scanner capable of scanning both small and large slides.

The NanoZoomer S60 supports both brightfield and fluorescence imaging. Brightfield and fluorescence images of whole tissues can be superimposed and viewed.

The NanoZoomer S60 uses an sCMOS camera for scientific measurement to capture precise fluorescence images, resulting in improved image quality with high sensitivity and shorter scanning time compared to the conventional NanoZoomer series.